![]() ![]() ![]() The further verification test demonstrated that Md MYB46 could activate their transcription by directly binding to the promoters of these genes. To explore whether Md MYB46 could coordinate stress signal transduction pathways to cooperate with the formation of secondary walls to enhance the stress tolerance of plants, Md ABRE1A, Md DREB2A and dehydration‐responsive genes Md RD22 and Md RD29A were screened out for their positive correlation with osmotic stress, salt stress and the transcriptional level of Md MYB46. ![]() In transgenic Arabidopsis and apple, Md MYB46 promoted the biosynthesis of secondary cell wall and deposition of lignin by directly binding to the promoter of lignin biosynthesis‐related genes. The Md MYB46 transcription factor was identified, and the stress treatment test of Md MYB46‐overexpressing and Md MYB46‐ RNAi apple lines indicated that Md MYB46 could enhance the salt and osmotic stress tolerance in apple. To expand the cultivation area of apple ( Malus×domestica Borkh.) and select resistant varieties by genetic engineering, it is necessary to clarify the mechanism of salt and osmotic stress tolerance in apple. ![]()
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